Preheat your oven to 350°F (175°C). Line a rimmed baking sheet or a loaf pan with parchment paper or lightly grease it to prevent sticking.

In a large mixing bowl, combine the ground beef, minced garlic, chopped onion, eggs, bread crumbs, Worcestershire sauce, milk, Italian seasoning, salt, and pepper. Mix gently until well combined, but avoid overmixing.

Transfer the meat mixture onto the prepared baking sheet or into the loaf pan, shaping it into a loaf.

In a small bowl, mix together the ketchup, brown sugar, and mustard to prepare the glaze.

Spread half of the glaze over the top of the meatloaf. Bake in the preheated oven for 40-45 minutes or until the internal temperature reaches 160°F (71°C).

During the last 10 minutes of baking, spread the remaining glaze over the meatloaf.

Once baked, remove the meatloaf from the oven and let it rest for 10 minutes before slicing.